Isolation of Bacillus amyloliquefaciens NTBA and determination of the growth-promoting activity on Chinese cabbage
To explore excellent strains of growth promoting bacteria,endophytic bacteria isolated from tobacco were iden-tified using morphological and molecular biology methods,and its growth promoting activity on Chinese cabbage was determined.The endophytic bacterium was identified as Bacillus amyloliquefaciens,and assigned as NTBA.It was found that the bacterium had the activity of hydrolyzing organic phosphorus,inorganic phosphorus and potassium,and could produce IAA.The growth promotion effect on Chinese cabbage was determined by pouring 100 μL,10 μL and 1 μL bacterial solutions in the soil and by soaking Chinese cabbage seeds with bacterial solution,with sterile water as the control.The results showed that after cultured for 10,15,and 20 days,the leaf length,leaf width,plant height,and stem diameter of Chinese cabbage treated with the two methods were significantly higher than those of the control.After 20 days of culture,compared with the control,the fresh mass of Chinese cabbage treated by pouring three concentrations of the bacte-rial solution and soaking seeds with bacterial solution increased by 78.99％,76.08％,70.29％,and 84.78％,respectively.The dry mass increased by 86.44％,76.27％,31.36％,and 88.98％,respectively.The root growth of Chinese cabbage was increased by 118.98％,83.94％,45.99％,and 63.50％,respectively.In conclusion,B.amylolyquenfaciens NTBA can obviously promote the growth of Chinese cabbage and increase its yield.This study provides beneficial plant growth-pro-moting bacteria for the production of Chinese cabbage and potential growth-promoting bacteria resources for other crops.
万方数据
维普
