Identification of seed purity of watermelon hybrid Xinxi 3 by SSR marker
In order to improve the efficiency of hybrid screening,this study screened specific primers suitable for the wa-termelon hybrid Xinxi 3 and its parents from 23 pairs simple sequence repeat(SSR)primers in watermelon.The results showed that one pair of primers BVWS00208 showed polymorphism between parents and had both parental bands in hy-brid offspring,belonging to codominant marker.The primer was validated in 98 hybrid individuals,and the purity identifi-cation result was 98.9％.The traditional field test showed the purity was 98.0％,with an error of ≤1％between them.This indicates that the primer BVWS00208 can serve as a specific primer for seed purity detection of watermelon hybrid Xinxi 3.The identification is convenient and economical,which can provide technical support for the seed production and promo-tion of this variety.
万方数据
维普
