利用SSR标记鉴定西瓜杂交种新喜3号纯度

Identification of seed purity of watermelon hybrid Xinxi 3 by SSR marker

任艺慈 ¹刘喜存 ¹王文英 ¹高展 ¹王向东 ¹郭春江 ¹李江伟²

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作者信息

1. 新乡市农业科学院河南新乡 453000
2. 新乡市农产品质量安全检测检验中心河南新乡 453000
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摘要

为了提高杂交种筛选效率,从23对西瓜简单重复序列(simple sequence repeat,SSR)引物中筛选适用于西瓜杂交种新喜3号及其亲本的特异性引物并进行种子纯度鉴定.结果表明,1对引物BVWS00208在亲本间具有多态性且在杂交后代中兼具双亲条带,属于共显性标记.将该引物在98个杂交后代中进行验证,纯度鉴定结果为98.9％,通过传统田间纯度鉴定结果为98.0％,二者误差≤1％.综上,SSR引物BVWS00208能够作为特异引物对西瓜杂交种新喜3号进行种子纯度鉴定,且该方法便捷经济,能为该品种的制种和推广提供技术支持.

Abstract

In order to improve the efficiency of hybrid screening,this study screened specific primers suitable for the wa-termelon hybrid Xinxi 3 and its parents from 23 pairs simple sequence repeat(SSR)primers in watermelon.The results showed that one pair of primers BVWS00208 showed polymorphism between parents and had both parental bands in hy-brid offspring,belonging to codominant marker.The primer was validated in 98 hybrid individuals,and the purity identifi-cation result was 98.9％.The traditional field test showed the purity was 98.0％,with an error of ≤1％between them.This indicates that the primer BVWS00208 can serve as a specific primer for seed purity detection of watermelon hybrid Xinxi 3.The identification is convenient and economical,which can provide technical support for the seed production and promo-tion of this variety.

关键词

西瓜/新喜3号/SSR分子标记/纯度鉴定

Key words

Watermelon/Xinxi 3/SSR molecular marker/Purity test

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出版年

2024

中国瓜菜

中国农业科学院郑州果树研究所

中国瓜菜

北大核心

影响因子：0.452

ISSN：1673-2871

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