Development of melon KASP markers and construction of fingerprints
To enrich spectrum of molecular markers in melon and offer a straightforward and efficient protocol for the sub-sequent identification of melon germplasm,variety,and seed purity,this investigation identified six pivotal single-nucleo-tide polymorphism(SNP)loci distributed across the melon genome,encompassing a total of 72 markers.Utilizing the KASP(kompetitive allele-specific PCR)assay,these markers were employed to genotype 11 melon germplasm acces-sions,resulting in the identification of 45 marker pairs exhibiting superior typing performance.The success rate of marker design reached 62.5％,and from these 45 pairs,the most optimal markers for the 25 identified genetic clusters were select-ed,and the polymorphisms of 31 muskmelon germplasm were divided into 3 groups,and the fingerprints of 31 muskmel-on germplasm were constructed to provide reference for the utilization of muskmelon germplasm resources and seed puri-ty identification.
万方数据
维普
