To investigate the antibacterial effects of different pesticides on Fusarium asiaticum,the pathogen of fruit rot disease in melon,and to clarify the effects of synergistic application of different chemical and microbial agents on the rhizosphere soil microbial community,the mycelial growth rate method was employed to determine the sensitivity of F.asiaticum to different pesticides,and the effects of different chemical and microbial agents on the structure of rhizosphere soil microbial communities were analyzed using metagenomic sequencing.The results showed that 50％prochloraz manga-nese salt WP had a significant inhibitory effect on the growth of F.asiaticum hyphae,with an EC50 value of 0.03 mg·L-1.Based on metagenomic sequencing analysis,the synergistic treatment of different chemical and microbial agents resulted in significant differences in the diversity of rhizosphere soil microorganisms,while significantly increasing the abundance of beneficial bacterial communities such as Actinobacteriota and Cladosporium.The synergistic treatment of soil fumi-gant"Mianlong"+polyglutamic acid organic fertilizer+Gulefeng 8.8 billion microbial agents has improved the richness and diversity of rhizosphere soil microorganisms,and the field control effect on the pathogenic Fusarium spp.is the best in the treatment group.The research results revealed the effects of synergistic application of different chemical agents and mi-crobial agents on soil microbial communities,which can provide reference for the field control of Fusarium spp.in melon.
关键词
甜瓜果腐病/药剂敏感性/化学药剂/微生物菌剂/土壤微生物
Key words
Melon fruit rot disease/Drug sensitivity/Chemical agents/Microbial agents/Soil microorganism
维普
万方数据