Genome-wide identification and expression analysis of the strictosidine synthetase genes in tomato
As a key enzyme in monoterpene indole alkaloids biosynthesis,strictosidine synthetase(STR)plays a decisive role in the synthesis of indole alkaloids.At present,there are some reports about the STR genes function in Camelliasinen-sis,Arabidopsis thaliana and other plants under adversity stress.But the related studies have not been reported in tomato.In order to study the function of STR genes in tomato,14 strictosidine synthase genes(SlSTR1-14)were identified from to-mato genome,and their physicochemical properties,gene structure,phylogenetic evolution and expression patterns were also analyzed.The result of physicochemical analysis showed that all S1STR except SlSTR8,9,11,12 and 14,were hydro-philic proteins,and subcellular localization prediction showed that all S1STR were located in vacuoles.Phylogenetic anal-ysis showed that STR famliy genes were unevenly distributed in Ⅵ groups of Solanum lycopersicum,Arabidopsis,Oryza-sativa and Camelliasinensis.Cis-acting element analysis showed that the promoter regions of SlSTR genes contain several regulatory elements related to stress and hormone responses.The results of gene expression analysis showed that SlSTR genes were mainly expressed in the stem,leaf and flower tissues of tomato.Drought and high-temperature treatments were applied to tomato seedlings,and the results showed that,compared to before treatment,the expression levels of most genes significantly increased after treatment.However,a few genes such as SlSTR1,2,and 8 showed significantly de-creased expression levels after drought treatment,while SlSTR8,11,and 12 showed significantly decreased expression lev-els after high-temperature treatment.This indicates that the expression patterns of the STR gene family are different when facing different adversity stresses.The results of this study identified the physicochemical information of STR genes in to-mato,and provided the direction for further research,the specific gene function still needs further verification.
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