Molecular identification of mosaic disease on Houttuynia cordata Thumb.in Dangyang
The identification of pathogenic bacteria species causing Houttuynia cordata mosaic disease in Dangyang will provide a theory basis for the field control of the disease.The mosaic leaves of Houttuynia cordata were collected to ex-tract the total RNA of typical mosaic disease samples,and sRNA deep sequencing was conducted.Based on bioinformat-ics analysis,the pathogen was initially identified.Using the virus coat protein(CP)specific primers for RT-PCR detection,the obtained sequences were subjected to BLAST alignment and a phylogenetic tree construction to further identify the pathogen.Using sRNA deep sequencing and bioinformatics analysis,it was initially determined that the causative virus of Houttuynia cordata mosaic disease was cucumber mosaic virus(CMV).Specific primers amplified a specific band of 878 bp,which was found to be highly homologous to published CMV sequences through BLAST alignment,with a similarity of 97.84％.By constructing a phylogenetic tree based on the nucleotide sequence of CMV virus CP gene,it can be con-cluded that Houttuynia mosaic virus belongs to subgroup Ⅱ of CMV.This result provides a theoretical basis for the pre-vention and treatment of Houttuynia cordata mosaic disease.
万方数据
维普
