Alleviation mechanism of exogenous nitric oxide on heat stress of Lenti-nus edodes
To investigate the mechanism of exogenous NO alleviating heat stress in Lentinula edodes,L.edodes strain L135 was used as the experimental material to study the alleviating effect of exogenous NO on oxidative damage of L.edodes hyphae under high temperature stress.The results show that high temperature stress significantly inhibited the growth of L.edodes mycelia,caused mycelia curl or even break,SNP treatment with appropriate concentration(800 pmol·L-1)could effectively alleviate the morphological damage caused by heat stress;the levels of H2O2 and MDA content in myceli-al cells decreased significantly in the group treated with exogenous SNP compared with the group treated without SNP,heating shock 48 and 72 h decreased by 17.48％,35.24％and 16.70％,19.87％respectively,and the intracellular activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activity were significantly increased.In conclusion,the addition of 800 μmol·L-1 exogenous NO can effectively alleviate heat stress in L.edodes,laying a theoretical founda-tion for further elucidating the mechanism of high-temperature stress resistance in edible fungi.
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