Study on the degradation and utilization of lignocellulose in cultivation materials of Lentinus edodes at different growth stages
In order to deeply understand the characteristics of lignocellulose variation in Lentinus edodes culture during the growth and development periods,the lignocellulose content and its related degrading enzyme activity in cultivation materials of L.edodes at different growth stages were determined.The changes of molecular groups of lignocellulose,rela-tive crystallinity index(CrI)of cellulose and fiber surface morphology were also studied and observed by Fourier trans-form infrared spectroscopy(FTIR),X-ray diffraction(XRD),and microscope.The results showed that the content of cel-lulose,hemicellulose and lignin decreased significantly during the growth of L.edodes.The content of lignocellulose de-creased from 85.27％to 52.23％,which was negatively correlated with the activity of the corresponding degrading en-zyme,and the degradation of lignocellulose showed a certain correlation with the degree of fiber damage.The FTIR re-sults showed that the absorbance of characteristic functional groups of lignocellulose in different growth stages had changed,and the degradation rate of lignin was higher than hemicellulose and lower than cellulose.With the growth of L.edodes,the CrI in cultivated materials decreased significantly,and the crystal structure of cellulose was destroyed to some extent.The results showed that the L.edodes was used the non-lignocellulosic components in cultivation materials for my-celium growth firstly,and secreted extracellular degrading enzymes to destroy the fiber structure and degrade the macro-molecular components into small molecular components at the same time,thus meeting its growth needs.The high activi-ty of lignocellulose degrading enzymes corresponds to the high degradation rate of lignocellulose,and the results can pro-vide a basis for improving the degradation and utilization of lignocellulose in L.edodes cultivation materials.
万方数据
维普
