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槟榔芋脱毒试管芋的诱导及种苗繁育研究

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为解决槟榔芋组培脱毒苗育苗成活率低、周期长、成本高、栽培后植株生长缓慢、子芋数量过多等问题,以炭步槟榔芋组培继代增殖8代的试管苗为材料,开展试管芋诱导、育苗基质和育苗时间,以及在高糖无激素培养基中进行不同周期培养对内源激素含量及生产影响的研究.结果表明,广东炭步槟榔芋单株试管苗在无激素培养基中,诱导试管芋的最佳蔗糖质量浓度为60g·L-1;育苗基质以红壤土、泥炭土、珍珠岩/椰糠/蛭石体积比2∶1∶1为最佳,成活率可达100%;试管芋的最佳育苗时间为11月份,此时可以最大程度地保证种苗的大小,降低生产成本,且能避免冬季低温影响种苗成活率.试管苗在高糖无激素培养基中,随着培养周期的延长,试管芋随之增大,脱落酸(ABA)含量升高,生长素(IAA)含量先升高后降低,细胞分裂素(CTK)含量先降低后升高再降低,在试管芋形成过程中起重要的调控作用.研究结果可为槟榔芋健康种苗繁育和规模化生产提供重要参考依据,也为芋球茎生长发育机制研究奠定基础.
Study on induction and seedling breeding of virus-free in vitro taro of Binglang taro
In order to solve the problems of low survival rate,long cycle,high cost,slow plant growth after cultivation,and excessive number of taro seeds in tissue culture virus-free seedlings of Binglang taro.This study used tissue culture and subculture of 8-generation in vitro seedlings of Binglang taro in Guangdong,Tanbu as experimental materials,in vitro taro induction,seedling substrate and seedling time were carried out.It also studied the endogenous hormone content and its impact on production after different transfer times in high sugar hormone free medium.The results showed that the op-timal sucrose concentration for inducing in vitro taro in hormone free medium was 60 g·L-1.The optimal seedling sub-strate is red loam∶peat soil∶perlite/coconut bran/vermiculite=2∶1∶1,and the survival rate can reach 100%.The best time for cultivating in vitro taro seedlings is in November,which can maximize the size of the seedlings,reduce production costs,and avoid the impact of low temperatures in winter on the survival of seedlings.In high sugar hormone free culture medium,as the number of transfers increases,the size of in vitro taro,the content of ABA increases,the content of IAA first increases and then decreases,and the content of CTK first decreases and then increases and then decreases.They play an important regulatory role in the formation process of test tube tubers.These results can provide important reference for the healthy seedling breeding and large-scale production of the Binglang taro,and lay a foundation for the study of the growth and development mechanism of the taro bulb.

Binglang taroIn vitro taroInductionSeedling breeding

黄绍力、罗燕羽、魏利国、莫现城、林荣善、李天明

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广州市农业科学研究院 广州 510335

贺州市农业农村局 广西贺州 542800

贺州市种子与种植业工作站 广西贺州 542800

槟榔芋 试管芋 诱导 种苗繁育

2024

中国瓜菜
中国农业科学院郑州果树研究所

中国瓜菜

北大核心
影响因子:0.452
ISSN:1673-2871
年,卷(期):2024.37(9)