In order to solve the problems of low survival rate,long cycle,high cost,slow plant growth after cultivation,and excessive number of taro seeds in tissue culture virus-free seedlings of Binglang taro.This study used tissue culture and subculture of 8-generation in vitro seedlings of Binglang taro in Guangdong,Tanbu as experimental materials,in vitro taro induction,seedling substrate and seedling time were carried out.It also studied the endogenous hormone content and its impact on production after different transfer times in high sugar hormone free medium.The results showed that the op-timal sucrose concentration for inducing in vitro taro in hormone free medium was 60 g·L-1.The optimal seedling sub-strate is red loam∶peat soil∶perlite/coconut bran/vermiculite=2∶1∶1,and the survival rate can reach 100％.The best time for cultivating in vitro taro seedlings is in November,which can maximize the size of the seedlings,reduce production costs,and avoid the impact of low temperatures in winter on the survival of seedlings.In high sugar hormone free culture medium,as the number of transfers increases,the size of in vitro taro,the content of ABA increases,the content of IAA first increases and then decreases,and the content of CTK first decreases and then increases and then decreases.They play an important regulatory role in the formation process of test tube tubers.These results can provide important reference for the healthy seedling breeding and large-scale production of the Binglang taro,and lay a foundation for the study of the growth and development mechanism of the taro bulb.
维普
万方数据