Study on the cold resistance of indoor and natural population of Epilach-na insignis in southern Henan province
In order to clarify the cold-resistance and low-temperature acclimation of Epilachna insignis in southern Henan province,the supercooling point and freezing point were determined by using the supercooling point meter,and the fre-quency distribution of the supercooling point was analyzed.The results showed that the supercooling point(-23.69 ℃)and freezing point(-21.31 ℃)of eggs were the lowest.The supercooling point(-6.77 ℃)and freezing point(-3.93 ℃)of the 4th instar larva were the highest,and there were significant differences in the supercooling point and freezing point between male and female adults of E.insignis.The supercooling point(-19.61 ℃)and freezing point(-17.67 ℃)of male adults were significantly lower than those of female adults(-14.23 ℃)and(-11.49 ℃),and the supercooling and freezing points of male and female adults in the indoor population were not significantly different from those in the natural population.The changes in supercooling point and freezing point of different individuals of the same instar and different stages were in accordance with normal distribution.The results of cold acclimation showed that the mortality of female adults increased gradually with the decrease of the treatment temperature.There was no significant difference in the super-cooling point among the treatments,and the lowest freezing point(-13.99 ℃)was at 8 ℃.