Research progress on molecular biological detection of edible mushroom strains
In recent years,edible mushroom industry of China has developed rapidly.But the problems such as confusion and unclear source of edible mushroom strains have become important issues restricting the development of the industry.Classic identification methods of edible mushroom strains such as morphologic characteristic observation,analysis of variety antagonistic reaction are time-consuming and inaccurate sometimes.The continuous progress of molecular biology technology has provided strong technical support for the development of edible mushroom industry and facilitates the detection of edible mushroom strains,variety identification and genetic resource conservation.The molecular methods of edible mushroom strains are DNA marker method,DNA sequence analysis and DNA barcoding.DNA marker techniques include the methods based on molecular hybridization such as RFLP,DNA fingerprinting methods based on PCR technique such as RAPD and novel markers based on the genome sequencing such as SNP and MNP.DNA sequence analysis technique is the analysis of ITS,IGS and the analysis based on genome sequencing.DNA barcoding technique includes the ITS barcoding and house-keeping gene barcoding such as EF-1α,β-tubulin.In this paper,the research progress on the molecular biological detection of edible mushroom strains in recent years was reviewed,and prospects for future molecular detection of edible mushroom strains was addressed,which provided reference for rapid and accurate identification of mushroom strains and variety breeding.
Mushroom strains authenticity detectionMolecular markerVariety identificationMachine learningCRISPR/Cas system
万方数据
维普
