Identification and expression analysis of TALE gene family in watermelon
The TALE gene family plays a pivotal role in regulating various aspects of plant growth and development,including floral organ development,floral meristem formation,organ morphogenesis,and fruit development,as well as mediating plant responses to abiotic stress and hormone signals.This study utilized bioinformatics methods to identify members of the TALE gene family in watermelon and conducted a comprehensive analysis of their gene structure,phylo-genetic relationships,conserved motifs,cis-acting elements,tissue-specific expression patterns,and gene expression pro-files under low temperature and drought stress conditions.This analysis provides a theoretical foundation for further functional studies and utilization of the TALE gene family in watermelon.The results revealed that the watermelon TALE gene family consists of a total of 19 members.Phylogenetic analysis classified the ClTALE family into two distinct subfamilies:BELl-like and KNOX,with the KNOX subfamily further subdivided into two classes,Class Ⅰ and Class Ⅱ.Cis-acting element analysis demonstrated that the promoters of ClTALE genes are enriched in cis-elements that respond to light,plant hormones,and abiotic stresses,with light-responsive elements being the most prevalent.Tissue-specific expression analysis in watermelon indicated that ClBLH09 is highly expressed in roots,while ClBLH01 exhibits high expression levels in tendrils,female flowers,and male flowers,implying that the watermelon TALE gene family may be involved in the growth and development of watermelon.Gene expression analysis at various time points under low tem-perature stress(0,3,6,12,24,36,and 48 h)and drought stress(0,1,2,3,4,5,6,7,and 8 d)revealed that ClBLH08 and ClKN02 exhibited an upward trend in expression under low temperature stress,while ClBLH04 and ClBLH05 showed an upward trend under drought stress.These findings suggested that the watermelon TALE gene family may play a signifi-cant role in mediating responses to low temperature and drought stress.
万方数据
维普
