Cloning and expression analysis of CmoAux/IAA gene in Cucurbita mos-chata
In the previous study,transcriptomic analysis of abortive pistil and normal pistil of Cucurbita moschata found that CmoAux/IAA gene might be involved in female flower development.In order to further explore its biological func-tion,a 600 bp gene(CmoAux/IAA)was cloned from Cucurbita moschata by homologous sequence cloning method and it encodes 199 amino acids.The results of conserved domain analysis showed that it belongs to the Aux/IAA family and includes domains Ⅰ,Ⅲ,and Ⅳ.The protein has no signal peptide and transmembrane structure.Multiple sequence align-ment and phylogenetic tree results showed that the amino acid sequence of CmoAux/IAA were closely related to XP_022986212.1 of Cucurbita maxima and XP_023513171.1 of Cucurbita pepo,and the similarity was 98.01％and 96.50％,respectively.The results of subcellular localization of Arabidopsis protoplasts showed that the protein was located in nucleus and cytoplasm.Real-time fluorescence quantitative results showed that CmoAux/IAA has tissue expression speci-ficity,mainly expressed in the growing points and stigma of female flowers in pumpkin plants,and the expression level of CmoAux/IAA in stigma was 137 fold that of in stamen,but not expressed in leaves and roots and it may play an important role in the differentiation of young tissue or the development of female flowers.
万方数据
维普
