The aim of this study was to select a low-cost composite substrate formulation suitable for cultivate high-quality Luffa seedling in cavity trays and hydroponics seedling in Hunan region,providing a theoretical and practical basis for Luffa seedling cultivation.Using commercial seedling growing medium as control(CK),11 different proportions of substrates were prepared from the raw materials of peat,vermiculite and perlite.Two methods of seedling cultivation were used,namely hole tray and floating seedling cultivation.After the seedling cultivation,the morphological characteristics of different treatments of Luffa seedling were observed,and their agronomic traits and physiological indicators were deter-mined.And the membership function method was used for comprehensive evaluation.The results showed that the physi-cochemical properties of the composite substrates with different ratios basically met the requirements of seedling nursery substrates.Using principal component analysis to comprehensively analyze the various indicators of seedlings and calcu-late the comprehensive score,the comprehensive ranking of various indicators of Luffa seedling under hole tray nursery was CK>T1>T10>T8>T7>T3>T6>T11>T2>T9>T4>T5,and that under floating nursery was T11>T1>CK>T6>T9>T3>T10>T7>T8>T2>T5>T4.The optimal composite substrate ratio for Luffa was T1(V grass charcoal∶V vermiculite∶V perlite=1∶1∶1)in hole tray nursery seedling and T11(Vgrasschareoal∶V vemiculite∶V perlte=3∶2∶2)in floating seedling.