Layan 509 was a new three-line hybrid fresh-eating pepper cultivar bred by male sterile y92-1A as the female parent and GJH-4-2① as the male parent.The cultivar belongs to mid-early maturity,the fruit type is line,single and downward,the first flower has an average of 8 nodes,and the total growth period is about 179 days.The young fruit is green while the mature fruit is bright red,the fruit surface is smooth light and microfold.The vertical diameter of the fruit is 20.36 cm,the horizontal diameter is about 1.83 cm,the average flesh thickness is 0.148 cm,the single fresh fruit mass is 14.58 g.Vitamin C content was 173 mg·100 g-1,and capsaicin content was 0.15%.Layan 509 had resistance to anthrax,resistance to cucumber mosaic virus disease,tobacco mosaic virus disease,anthracnose,medium resistance to blight.The average yield of fresh pepper was 1 547.65 kg·667 m-2,which showed it has stable high yield.It is suitable for protected and outdoor planting in Guizhou,Ningxia,Xinjiang and other places.