Analysis of endogenous hormones content and related gene expression in reverse thermo-sensitive genic male sterile line of eggplant
Male sterility is crucial for hybrid breeding,and digging key candidate genes for male sterility of eggplant is helpful to directional improvement of male sterile materials. In this experiment,using the thermo-sensitive male sterile line 05ms and the fertile line S63 as experimental materials,transcriptome sequencing was performed on the anthers of sterile line 05ms and fertile line S63 at meiosis stage(Ⅱ),and the endogenous hormone contents of the pollen mother cell stage(Ⅰ),meiosis stage(Ⅱ),microspore stage(Ⅲ)and mature pollen grain stage(Ⅳ)of 05ms under low-temperature sterility period(ML)in spring and high-temperature fertility period(MH)in summer were detected. The results showed that differential expressed genes were mainly concentrated in the metabolic pathways of pentose and gluconate conver-sion,carotenoid biosynthesis and plant hormone signal transduction. The key regulatory genes of thermo-sensitive male sterility in eggplant,such as Smechr0702283,Smechr0801802,Smechr0600105,Smechr0802213,Smechr0302844,Smechr0601316,Smechr0401804,were identified by qRT-PCR,which confirmed the reiliability of the transcriptome data. The results of hormone metabolite detection showed that the content of auxin hormone ICAld and ethylene precursor ACC in ML were significantly higher than those in MH at all of the four stages,while the content of auxin IAA in ML were lower than those in MH. The author analyzed the mechanism of anther abortion in eggplant at the metabolism and transcription level,which laid a theoretical foundation for further revealing the regulatory mechanism of thermo-sensitive male sterility in eggplant.
EggplantTranscriptomicMetabolomicThermo-sensitive male sterilityHormone
万方数据
维普
