首页|蜜瓜籽不同溶剂提取物活性成分及抗氧化能力分析

蜜瓜籽不同溶剂提取物活性成分及抗氧化能力分析

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以河套地区5个不同品种蜜瓜籽(华莱士、银蜜、西州密25号、玉妃和红蜜)为研究对象,分别采用蒸馏水、70%乙醇、70%丙酮等不同溶剂进行提取,测定其总黄酮、总多酚和总多糖含量并与抗氧化活性进行相关性分析.结果表明,不同溶剂提取物抗氧化效果不同,70%乙醇提取物清除DPPH自由基、ABTS+自由基和抗氧化还原能力最强;华莱士瓜籽70%乙醇提取物中总黄酮和总多酚含量(w,后同)最高,分别为94.74、35.90 mg·g-1;总多糖含量最高的是华莱士瓜籽水提取物,达159.29 mg·g-1.相关性分析表明,总多酚含量与DPPH自由基清除能力、ABTS+自由基能力和还原能力呈显著或极显著正相关,总黄酮含量与DPPH自由基清除能力、还原能力呈显著或极显著正相关.建议将70%乙醇作为河套蜜瓜籽的提取溶剂,为河套蜜瓜副产物的进一步开发、提取和利用提供理论依据.
Active ingredients and antioxidant capacity analysis of different solvent extracts from melon seeds
In this study,distilled water,70% ethanol and 70% acetone respectively were used as solvent to extract flavo-noids,polyphenols and polysaccharides from five different cultivars of melon seeds(Hualaishi,Yinmi,Xizhoumi,Yufei and Hongmi). The correlation analysis between bioactive components and their antioxidant ability were explored. The results showed that fractions extracted by 70% ethanol from all samples expressed the best anti-redox capacity by significantly scavenging DPPH and ABTS+radicals. Moreover,fractions extracted by 70% ethanol from Hualaishi melon seeds had the highest amount of total flavonoids(94.74 mg·g-1)and total polyphenols(35.90 mg·g-1)and fractions ex-tracted by distilled water from Hualaishi melon seeds had the highest amount of total polysaccharides(159.29 mg·g-1). The results of correlation analysis showed that the amount of total flavonoids and total polyphenols had significant positive correlations with the ability of scavenging DPPH,ABTS+radicals and anti-redox. In conclusion,70% ethanol was a potential preferences for extracting bioactive components from melon seeds and the findings would establish a theoretical basis for further research of by-products produced by Hetao melon.

Melon seedAntioxidant activityTotal flavonoidsTotal polyphenolsTotal polysaccharides

刘聪、王吉力特、孙义、苏日古格、马捷、张凡、郭淑文

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河套学院农学系 内蒙古巴彦淖尔 015000

巴彦淖尔市农牧业科学研究所 内蒙古巴彦淖尔 015000

蜜瓜籽 抗氧化活性 总黄酮 总多酚 总多糖

2024

中国瓜菜
中国农业科学院郑州果树研究所

中国瓜菜

北大核心
影响因子:0.452
ISSN:1673-2871
年,卷(期):2024.37(11)