Comparative analysis of root exudates between healthy and soft rot diseased Amorphophallus konjac
Soft rot is one of the most serious diseases in konjac cultivation,and there is currently no effective chemical agents. In order to understand the relationship between the changes of konjac root exudates induced by soft rot disease,the differences of root exudates between healthy(HK)and soft rot diseased konjac(DK)were analyzed by high-perfor-mance liquid chromatography tandem mass Fourier transform(UHPLC-q Exactive HF-X). The results showed that the root exudates of healthy and diseased konjac were significantly different,and 340 different metabolites were identified. There were 9 special metabolites in HK,among which phenylpropane and polyketone compounds were helpful to im-prove the disease resistance of plants. There were 7 special metabolites in DK,among which maltohexaose and lacto-N-fu-copentaose Ⅲ contributed to the recognition and infection of soft rot bacteria in konjac roots. Cluster analysis of differen-tial metabolic pathways revealed that there were 212 metabolites related to metabolic pathways,among which the tricar-boxylic acid cycle of citric acid was up-regulated in HK,higher than in DK. The research results provide a theoretical ba-sis for the use of secondary metabolites in root for soft rot prevention and control.
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