Selection of suitable Lentinula edodel strains for summer cultivation in Anlong,Guizhou province
In order to select suitable Lentinula edodels strains for summer cultivation in Anlong county,six L.edodels strains collected from various L.edodels strains growing enterprises in Anlong county were used as test materials,and the mycelial growth rate,antagonistic response,gene sequence,and single rod yield of the substrate of the six L.edodels strains were compared,and phylogenetic tree was constructed.The results showed that among the six L.edodels strains,the growth rate of the AL3 strain was faster,with an average growth rate of 4.51 mm·d-1,which was significantly higher than that of the other strains,followed by that of the AL6 strain,with an average growth rate of 4.37 mm·d-1;antagonism between the AL1 and AL6 strains and the other strains was obvious,and the rest of the strains had no antagonism that was not significant or antagonistic;ITS sequence alignment and phylogenetic analyses placed the six L.edodels strains into three major evolutionary categories of the Lentinula,the six strains were categorized into two homozygous strains and four different subspecies.AL4 strain had the highest single rod yield of 0.58 kg,followed by AL6 strain with single rod yield of 0.47 kg.The results of the experiments showed that among the six L.edodels strains,AL4,AL6 and AL3 strains were more suitable for summer cultivation in Anlong country,which provided theoretical support for the selection of suit-able L.edodels strains germplasm resources for summer cultivation in Anlong county.