Evaluation of the safety of four insecticides on mycelium and substrate of Pleurotus ostreatus
The objective of this study is to examine the impact of four insecticides,specifically diflubenzuron,dinotefu-ran,beta-cypermethrin and chlorantraniliprole,on the growth of the mycelium and fruiting bodies of Pleurotus ostreatus.The pharmacological treatment of P.ostreatus employs both mixing and spraying techniques.The residues were subjected to analysis using liquid chromatography-mass spectrometry(LC-MS/MS)and gas chromatography(GC).A study was conducted to evaluate the efficacy of four insecticides against dipteran pests,specifically mosquitoes and flies.The results demonstrated that the highest inhibitory efficacy of the four insecticides on the mycelium of P.ostreatus was 14.39% for chlorantraniliprole.The remaining three agents exhibited lower inhibition rates.The impact of diflubenzuron in conjunc-tion with beta-cypermethrin in the mixing treatment on the production of P.ostreatus substrates was found to be insignifi-cant.The initial flush of mushrooms was subjected to a bioconversion process,resulting in a conversion rate of over 56%.The application of beta-cypermethrin and dinotefuran in spray treatments has been observed to exert a minimal influence on the yield of P.ostreatus.The bioconversion rate of the initial flush of mushrooms was in excess of 57%.Dinotefuran has been demonstrated to be the most effective chemical agent against mosquitoes and flies,which are classified as dipter-an pests.The rate of population reduction can reach as high as 97.1%.The analysis of the treated substrates of P.ostreatus revealed the absence of beta-cypermethrin and chlorantraniliprole pesticide residues.Residue limits for all four insecti-cides tested were not exceeded in sprayed substrates.Overall,beta-cypermethrin can be used for mixing treatment of P.os-treatus,and dinotefuran can be used for spraying treatment.The impact on the safety of P.ostreatus substrate products is minimal.