Correlation between the expression of microRNA-21 and microRNA-223 in peripheral blood mononuclear cells and the efficacy of antiretroviral therapy in human immunodeficiency virus/acquired immune deficiency syndrome patients
Objective To investigate the relationship between the efficacy of antiretroviral therapy and the expression levels of microRNA-21(miR-21)and microRNA-223(miR-223)in peripheral blood mononuclear cells(PBMCs)in patients with human immunodeficiency virus(HIV)/acquired immune deficiency syndrome(AIDS).Methods A total of 142 HIV/AIDS patients who were treated with antiretroviral therapy in the outpatient department of Nantong Third People's Hospital from March 2020 to March 2022 were selected as the observation group.According to efficacy,they were grouped into effective group(n=110)and ineffective group(n=32).139 healthy people participated in medical examinations in the same period were selected as the control group.The expression levels of miR-21 and miR-223 in PBMCs were detected by real-time fluorescence quantitative polymerase chain reaction(PCR).Multivariate Logistic regression was applied to analyze the influencing factors of ineffectiveness of antiretroviral therapy in HIV/AIDS patients.Results The expression levels of miR-21 and miR-223 in PBMCs in the observation group were higher than those in the control group(P＜0.05).The expression levels of miR-21 and miR-223 in PBMCs of the ineffective group were higher than those of the effective group 3 months,6 months and 12 months after treatment(P＜0.05).In the effective group,the expression levels of miR-21 and miR-223 in PBMCs decreased in turn with the prolongation of treatment time(P＜0.05).The proportion of patients in World Health Organization(WHO)stage 3 before treatment in the ineffective group was higher than that in the effective group(P＜0.05).MiR-21,miR-223 and WHO stage 3 before treatment were independent risk factors that affected the ineffectiveness of antiretroviral therapy in HIV/AIDS patients(P＜0.05).Conclusions MiR-21 and miR-223 can reflect the efficacy of antiretroviral therapy in HIV/AIDS patients to a certain extent.The expression levels of miR-21 and miR-223 in PBMCs of patients with ineffective treatment are relatively high.
Human immunodeficiency virus/acquired immune deficiency syndromeAntiretroviral therapyPeripheral blood mononuclear cellsMicroRNA-21MicroRNA-223
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维普
