Inhibitory effect of luteolin on proliferation,migration,invasion and epithelial mesenchymal transformation of cervical cancer cells by blocking p38 mitogen-activated protein kinase pathway
Objective To explore the influence of luteolin on proliferation,migration,invasion and epithelial mesenchymal transformation(EMT)of cervical cancer cells by regulating p38 mitogen-activated protein kinase(MAPK)pathway.Methods Cervical squamous cell carcinoma SiHa cell line was treated with luteolin at different concentrations for 24 h,and cell viability was detected by cell counting kit-8(CCK-8)method to screen the optimal concentration of luteolin.Then,SiHa cells were divided into control group,luteolin group,positive drug group,inhibitor group and activator group.After 24 h of intervention,cell morphology,proliferation,migration,invasion and expression of EMT and p38 MAPK pathways related proteins were determined by inverted microscope,cell proliferation kit,wound healing,Transwell chamber and Western blotting.Results The optimal concentration of luteolin was determined to be 10 μmol/L by CCK-8 method.Compared with control group,the growth of cells was inhibited,cell proliferation rate,migration rate,invasion number,protein expression levels of Vimentin,N-cadherin and fibronectin(FN),p-p38 MAPK/p38 MAPK ratio were decreased(P＜0.05),while E-cadherin protein level was increased in the luteolin group and the positive drug group,compared with the control group(P＜0.05).SB203580 enhanced and anisomycin inhibited the above effects of luteolin on SiHa cells compared with luteolin group(P＜0.05).Conclusions Luteolin can inhibit the proliferation,migration and invasion of SiHa cells and the EMT process by blocking p38 MAPK pathway.
Cervical cancerLuteolinP38 mitogen-activated protein kinase signaling pathwayInvasionN-cadherin
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