Interaction between sperm parameters and DNA integrity and the effect of mycoplasma infection in male immune infertility patients
Objective To study the interaction between sperm parameters and DNA integrity and the effect of mycoplasma infection in male immune infertility patients.Methods A total of 120 male immune infertility patients diagnosed in Sichuan Jinxin Xinan Women and Children Hospital from January to December 2022 were retrospectively selected as the study group,and were divided into infected group(n=33)and non-infected group(n=87)according to whether they had mycoplasma infection.Another 120 normal males who underwent physical examination in the same hospital during the same period were selected as the control group.Semen quality and DNA integrity were compared between study group and control group,infected group and non-infected group,and the correlation between sperm DNA integrity and semen quality was studied.Results The semen liquefaction time in the study group was longer than that in the control group.Sperm motility rate,sperm concentration,forward motion grade and the normal morphology rate were lower than those in the control group.The semen liquefaction time in infected group was longer than that in non-infected group.Sperm motility rate,sperm concentration,forward motion grade and normal morph rate was lower than those in the non-infected group.In the study group,patients with no halo,small halo,medium halo,large halo and DNA fragment index were significantly higher than those in the control group.The patients in infection group with no halo,small halo,medium halo,large halo and DNA fragment index were significantly higher than those in control group.Correlation analysis showed that the semen liquefaction time was negatively correlated with large halo and DNA fragment index,and positively correlated with sperm motility rate,sperm concentration,forward motion grade and normal morphologic rate.Conclusions Sperm parameters and mycoplasma infection were significantly correlated with DNA integrity in male immune infertility patients,and mycoplasma infection may lead to a decrease in DNA integrity,thus affecting sperm quality.
Mycoplasma genitaliumMale infertilitySemen qualitySperm DNA integrity
万方数据
维普
