Value of early prediction of preeclampsia by using combination of maternal urine ceruloplasmin,uterine artery pulse index and pre-pregnancy body mass index
Objective To explore the predictive value of candidate proteins combined with uterine artery Doppler ultrasound and clinical risk factors for preeclampsia(PE).Methods A total of 60 pregnant women with PE who received prenatal examination and gave birth in Wuxi Maternity and Child Health Care Hospital from January 1 to December 31,2021 were selected as the study subjects,including 12 discovery sets and 48 verification sets.In addition,60 normal pregnant women without pregnancy complications in the same hospital during the same period were included as controls.The urine supernatant of all pregnant women was retained at 11-13+6 weeks of gestation,and the protein of which was detected by ultra-high performance liquid chromatogram-mass spectrometry(UPLC-MS)and enzyme-linked immunosorbent assay(ELISA).At the same time,the uterine artery pulse index(UtA-PI)was recorded by four-dimensional color Doppler ultrasound.The protein expression in placenta was analyzed by immunohistochemical staining.Results By UPLC-MS analysis,it was confirmed that ceruloplasmin was upregulated in the PE group of the discovery set and was used as a candidate protein for validation.In the validation set,the average UtA-PI and urine ceruloplasmin in early pregnancy were significantly higher in the PE group than in the normal pregnant group(P＜0.05).Urine supernatant ceruloplasmin,pre-gestational body mass index,and abnormal UtA-PI well predicted women with PE after 20 weeks of gestation,with sensitivity and specificity of 0.813 and 0.937,respectively.Furthermore,the level of ceruloplasmin in the urine supernatant was positively correlated with the mean integrated optical density value of ceruloplasmin in placental tissue(r=0.624,P＜0.001).Conclusions Urine ceruloplasmin may be a new biomarker for PE,and the combination of UtA-PI and clinical risk factors may contribute to the early prediction of PE.
PreeclampsiaUrine supernatantProteomicsCeruloplasminUterine artery pulse index
万方数据
维普
