Application value of time-difference culture in embryo selection in Hainan infertility population
Objective To establish of an embryo development dynamics parameter model based on time-difference culture technology and evaluate the value of the model in predicting high-quality blastocysts.Methods A total of 300 infertile female patients(1 000 embryos)undergoing in vitro fertilization-embryo transfer(IVF-ET)assisted pregnancy in Department of Reproductive Medicine of Hainan Modern Women's and Children's Hospital from October 2021 to June 2022 were selected as study objects.Embryos were cultured in vitro using the time-difference culture technology,and the dynamic parameters during the embryo development were recorded.Embryo transfer is performed after selecting blastocysts based on morphological scores.According to the number of high-quality blastocysts,they were divided into high-quality blastocysts group(n=74)and control group(n=384).The dynamic parameters of embryonic development between the two groups were compared,and multivariate Logistic regression equation was used to analyze the differences factors.The nomogram model was established using R language,and the receiver operating characteristic(ROC)curve evaluation model was used to predict the internal efficiency of high-quality blastocyst formation at an early stage.Results The tPNa of the high-quality blastocyst group was higher than that of the control group,and the levels of tPB2,t2,t3,t4,t5,and cc2 were lower than those of the control group,with statistical significance(P＜0.05).Multivariate Logistic regression analysis showed that tPNa(OR=1.396)was an independent risk factor for high-quality blastocyst formation(P＜0.05),while tPB2(OR=0.373),t3(OR=0.939),t4(OR=0.937),and cc2(OR=0.805)were independent protective factors for high-quality blastocyst formation(P＜0.05).The analysis of ROC curve showed that the area under curve(AUC)of the nomograph model of high-quality blastocyst formation based on independent related factors was 0.894,the sensitivity was 74.32％,and the specificity was 91.67％.Conclusions The time-difference culture technology can predict high-quality blastocyst formation at early stage by observing the dynamics parameters during embryo development and ensure clinical pregnancy outcome based on reducing the number of embryos transferred.
Time-difference cultureIn vitro fertilization-embryo transferDynamics parametersPrediction model
万方数据
维普
