Analysis of the relationship between peripheral blood histone deacetylase 2,programmed death ligand 1 protein,and regulatory T-cell levels with clinicopathological features and prognosis in patients with ovarian cancer
Objective To investigate the relationship between the detection of peripheral blood histone deacetylase 2(HDAC2)and programmed death ligand 1(PD-L1)protein and regulatory T-cell(Treg)detection with clinicopathological features and prognosis in patients with ovarian cancer.Methods A total of 60 OC patients diagnosed and treated at Zhangjiakou First Hospital from July 2018 to September 2019 were selected as the study group.The patients were grouped into survival group(n=43)and death group(n=17)according to the 3-year follow-up of patients'survival condition.Another 60 patients with benign ovarian tumors diagnosed in the same hospital during the same time were selected as the control group.The levels of Treg,T helper cell 17(Th17)and Th17/Treg level in peripheral blood of all patients were detected by flow cytometry,the levels of HDAC2 and PD-L1 proteins were detected by enzyme-linked immunosorbent assay.The relationship between the levels of Treg,Th17,Th17/Treg,HDAC2 and PD-L1 proteins and the clinicopathological features was analyzed,the peripheral blood Treg,Th17,Th17/Treg,HDAC2,and PD-L1 protein levels were analyzed and compared between the survival group and the death group after follow-up for 3 years after treatment.Logistic regression was applied to analyze the factors influencing the prognosis of OC patients.Results The levels of Treg,Th17,Th17/Treg,HDAC2 and PD-L1 in patients in the study group were significantly higher than those in the control group(P＜0.05).The levels of Treg,Th17,Th17/Treg,HDAC2 and PD-L1 proteins in OC patients in stage Ⅰ,stage Ⅱ,stage Ⅲ and stage Ⅳ increased successively,and the difference was statistically obvious(P＜0.05).The clinical stage,residual lesion size,lymph node metastasis,Treg,Th17,Th17/Treg,HDAC2 and PD-L1 proteins levels were all influencing factors for the prognosis of OC patients(P＜0.05).Conclusions Treg,Th17,Th17/Treg,HDAC2 and PD-L1 proteins participate in and affect the development of OC,which are closely related to the prognosis of patients.
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