Effect of different DNA fragmentation index levels of sperm in in vitro fertilization assisted pregnancy men on embryonic development and pregnancy outcome
Objective To investigate the effect of different DNA fragmentation index(DFI)of sperm in in vitro fertilization assisted pregnancy men on embryonic development and pregnancy outcome.Methods A total of 80 infertility couples who received in vitro fertilization(IVF)assisted pregnancy in Linfen People's Hospital from July 2020 to January 2022 and followed up to June 2023 were selected as the study subjects.According to the male sperm DFI level,they were divided into DFI＜25％group(n=44)and DFI≥25％group(n=36).Blastocyst formation rate,available blastocyst rate,dominant blastocyst rate,total sperm count,sperm concentration,forward motile sperm rate,forward motile sperm concentration,total forward motile sperm count,total immotile sperm count,blastocyst implantation rate,clinical pregnancy rate and abortion rate were compared between the two groups.Logistic regression model was used to analyze the effects of DFI on fetal development and pregnancy outcome.Results The blastocyst formation rate,available blastocyst rate and dominant blastocyst rate in DFI＜25％group were higher than those in DFI≥25％group(P＜0.05).The total sperm count,sperm concentration and total immotile sperm count in DFI＜25％group were lower than those in DFI≥25％group,but the rate of forward motile sperm,concentration of forward motile sperm and total forward motile sperm count were higher than those in DFI ≥25％group(P＜0.05).The blastocyst implantation rate and clinical pregnancy rate in DFI＜25％group were higher than those in DFI≥25％group(P＜0.05).There was no significant difference in abortion rate between the two groups(P＞0.05).Logistic regression analysis showed that DFI was the influencing factor of blastocyst formation rate,available blastocyst rate,dominant blastocyst rate,blastocyst implantation rate and clinical pregnancy rate(P＜0.05).Conclusions Different DFI levels of male sperm can affect the embryo development and pregnancy outcome of IVF assisted pregnancy patients.
In vitro fertilizationAssisting pregnancyMaleSperm DNA fragmentation indexEmbryonic developmentPregnancy outcome
万方数据
维普
