Effect of laser shrinkage blastocyst modes on clinical outcomes of freeze-thaw single blastocyst transfer
Objective To explore the effect of different laser shrinkage blastocyst modes(single-hole shooting and biopsy mode)on the outcomes of frozen-thawed single blastocyst transfer.Methods A total of 273 assisted-pregnancy patients who underwent frozen-thawed single blastocyst transfer in the Department of Reproduction,Handan Maternal and Child Health Hospital from September 2018 to August 2022 were selected as the study subjects.Based on the different laser shrinkage blastocyst modes before vitrification and freezing of the blastocyst,the patients were divided into the single-hole group(n=100)and the biopsy group(n=173).The condition of blastyocysts shrinkage before freezing,the revival rate of the freeze-thawed blastocysts,and the clinical pregnancy rate,multiple birth rate,and miscarriage rate after the freeze-thaw single blastocyst transfer were compared between the two groups.Results There were no statistically significant differences in the rates of complete shrinkage,partial shrinkage,and no shrinkage after laser shrinkage blastocyst modes between the two groups(P＞0.05).Additionally,there were no statistically significant differences in the blastocyst revival rate,clinical pregnancy rate,and miscarriage rate between the two groups(P＞0.05).The rate of multiple births in the biopsy group is slightly higher than that in the single-hole group,but the difference is not statistically significant(P＞0.05)Conclusions In order to reduce the potential thermal damage caused by the laser during blastocyst shrinkage to the trophoblast cells,the single-hole shooting mode can be selected for blastocyst shrinkage in multiple pregnancies after freeze-thaw blastocyst transfer.
Laser shrinkageVitrification and freezingFreeze-thawed blastocyst
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