Comparison of sperm detection bysperm microsurgical testicular sperm extraction pathological examination and testicular biopsy dissection examination in patients with non-obstructive azoospermia
Objective To investigate the consistency of sperm detection between microextraction of testis histopathology and testicular biopsy dissection examination in patients with non-obstructive azoospermia(NOA).Methods Retrospective analysis was performed on 197 NOA male patients who were admitted to the Affiliated Reproduction Hospital of Shandong University from January 2020 to December 2022.After sperm microsurgical testicular sperm extraction biopsy,testicular tissue pathology examination and testicular biopsy tissue dissection examination were performed,and the sperm retrieval rate was analyzed.Results According to the examination of testicular histopathology,patients were divided into non-spermatozoa group[spermatogenic arrest(MA)+Sertoli cell only syndrome(SCOS)]with 170 patients(86.3％)and spermatozoa group[hypospermatogenesis(HS)]with 27 patients(13.7％).Sperm were detected by testicular tissue dissection in 29 patients(14.7％),and spermatozoa were detected by testicular tissue dissection and intracytoplasmic sperm injection(ICSI)was performed in 7 SCOS patients.There was no significant difference in the sperm detection rate between the two methods(P＞0.05).Follicle-stimulating hormone(FSH)levels were significantly different between the two groups(P＜0.05).Conclusions The two methods has high consistency in finding sperm.The testicular biopsy pathological examination,as the etiology analysis,provides the basis for male physicians to predict the success of in vitro sperm extraction for NOA,FSH levels also help predict successful extraction.The spermatozoa found by testicular tissue dissection can be directly used in artificial assisted reproductive technology,so it is therefore more decisive.
Male infertilityNon-obstructive azoospermiaTesticular tissue pathological examinationTesticular biopsy dissection examination
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