Clinical effects of transforming growth factor-β1,interleukin-17 and interleukin-6 levels in seminal plasma on asthenospermia and acrosomal enzyme activity in patients with asthenospermia
Objective To observe the levels of transforming growth factor-β1(TGF-β1),interleukin-17(IL-17),interleukin-6(IL-6)and sperm acrosomal enzyme activity in the seminal plasma of patients with asthenospermia(AS),and to investigate the effects of seminal TGF-β1,IL-17 and IL-6 levels on AS and acrosomal enzyme activity in patients with AS.Methods A total of 110 patients with AS admitted to the Affiliated Panyu Central Hospital of Guangzhou Medical University from June to December 2022 were enrolled as study objects and set as the observation group.Another 51 healthy males participated in a health examination and had a normal semen examination were enrolled as the control group.The AS group was further divided into group A,group B and group C according to the percentage of progressive motility(PR).Semen routine parameters,acrosomal enzyme activity,seminal plasma TGF-β1,IL-17 and IL-6 levels were analyzed.Logistic regression was used to analyze the risk factors for AS,and linear regression was used to analyze the influencing factors of sperm acrosomal enzyme activity.Results Compared with the control group,seminal plasma levels of TGF-β1,IL-17 and IL-6 of group A,group B and group C were significantly increased(P＜0.05).Seminal levels of TGF-β1,IL-17,and IL-6 were negatively correlated with PR,total sperm motility[PR+percentage of nonprogressive motility(NP)],and sperm acrosomal enzyme activity(P＜0.01).Seminal level of IL-17 was negatively correlated with semen pH,semen volume and total sperm count(P＜0.05).Seminal plasma level of IL-6 was negatively correlated with total sperm count(P＜0.05).Levels of seminal plasma TGF-β1,IL-17 and IL-6 were associated with the occurrence of AS(P＜0.01).The increased seminal plasma levels of IL-17 and IL-6 lead to the decrease of acrosomal enzyme activity(P＜0.01).Conclusions The increased levels of TGF-β1,IL-17 and IL-6 in seminal plasma are risk factors for the occurrence of AS,and the increased levels of seminal plasma IL-17 and IL-6 in patients with AS are influencing factors leading to decreased sperm acrosomal enzyme activity.
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维普
