基于TCGA数据库分析RNF43在子宫内膜癌组织中的表达及意义
Analysis of expression and significance of RNF43 in uterine corpus endometrial carcinoma based on TCGA database
王仙婷 1蔡俊宏 2包珊1
作者信息
- 1. 海南医学院附属海南医院妇产科,海口 570311
- 2. 海南医学院附属海南医院中心实验室,海口 570311
- 折叠
摘要
目的 探讨RNF43在子宫内膜癌(UCEC)组织中的表达及潜在作用.方法 下载TCGA数据库中的UCEC组织(n=554)与癌旁组织(n=23)RNAseq数据及对应的临床数据,并利用R语言分析RNF43在不同临床病理特征UCEC中的表达.利用GEPIA 2在线数据库分析RNF43的表达与患者预后的关系;利用LinkedOmics在线数据库对RNF43及其共表达基因进行KEGG通路富集分析;利用R语言"limma"包分析RNF43与UCEC中免疫细胞的相关性.结果 RNF43在UCEC组织中的表达水平显著高于癌旁组织(P＜0.001).不同年龄、肿瘤分级患者RNF43的表达比较,差异具有统计学意义(P＜0.01).生存分析发现,UCEC患者中RNF43高表达组总生存期长于RNF43低表达组(P＜0.05).基因富集分析提示,RNF43与Wnt/β-Catenin/TCF信号通路、线粒体代谢、淋巴细胞及中性粒细胞调节的免疫相关(FDR＜0.05,P＜0.05).RNF43的表达与滤泡辅助性T细胞、活跃记忆CD4+T细胞、γδT细胞呈正相关(R＞0,P＜0.05),与中性粒细胞呈负相关(R＜O,P＜0.05).结论 RNF43可作为UCEC预后评估及免疫治疗的潜在标志物.
Abstract
Objective To investigate the expression and potential role of RNF43 in uterine corpus endometrial carcinoma(UCEC).Methods RNAseq data and corresponding clinical data of UCEC tissues(n=554)and adjacent tissues(n=23)were downloaded from TCGA database,and R language was used to analyze RNF43 expression in UCEC with different clinicopathological characteristics.The correlation between prognostic value of RNF43 and prognosis of patients was analyzed by GEPIA 2.The KEGG pathway enrichment analysis of RNF43 and its co-expressed genes was performed using the online database of LinkedOmics.The correlation between RNF43 expression and immune cells in UCEC was analyzed by using the R-package"limma".Results RNF43 expression in UCEC tissue was significantly higher than that in the adjacent tissues(P＜0.001).There were a statistically significant differencse in RNF43 expression among patients of different ages and tumor grades(P＜0.01).Survival analysis showed that the overall survival of UCEC patients with high expression of RNF43 was longer than that with low expression of RNF43(P＜0.05).Gene enrichment analysis suggested that RNF43 might be related to Wnt/β-Catenin/TCF signaling pathway,mitochondrial metabolism,lymphocyte and neutrophil mediated immunity.(FDR＜0.05,P＜0.05).The expression of RNF43 was positively correlated with follicular helper T cells,active memory CD4+T cells,γδT cells(R＞0,P＜0.05),and negatively correlated with neutrophils(R＜0,P＜0.05).Conclusions RNF43 can be used as a potential marker for prognosis and immunotherapy of UCEC.
关键词
子宫内膜癌/RNF43/预后/免疫细胞Key words
Uterine corpus endometrial carcinoma/RNF43/Prognosis/Immune cells引用本文复制引用
出版年
2024
NETL
NSTL
万方数据