Discussion on the effect mechanism of Kangfu Xiaoyan Suppository in the treatment of chronic prostatitis based on network pharmacology and molecular docking technology
Objective To explore the mechanism of Kangfu Xiaoyan Suppository in the treatment of chronic prostatitis based on network pharmacology and molecular docking technology.Methods The active ingredients and action targets of Kangfu Xiaoyan Suppository were obtained through traditional Chinese medicine systems pharmacology(TCMSP),UniProt,Huayuan network,Drug bank and other platforms combined with literature.The targets of chronic prostatitis were obtained from GeneCards,DisGeNET and PharmGKB databases.Venny 2.1.0 was used to obtain the common targets of drug and disease.The common targets were imported into the STRING platform to construct a protein interaction network.Cytoscape software was used to screen out the core targets and establish a"drug-core component-core target-disease"network.The DAVID platform was used to perform Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis of the core targets,and the pathways most related to chronic prostatitis were selected to establish the"key path-target"network and screen the key targets.Pymol and AutoDock Tools were used to perform molecular docking of the key targets and key components.Results A total of 315 targets of 150 active components of Kangfu Xiaoyan Suppository were obtained,and 250 common targets were obtained after intersection with 4327 disease targets.GO and KEGG analysis of 42 core targets showed that the biological processes were mainly positive regulation of RNA polymerase Ⅱ promoter transcription,positive regulation of DNA transcription,positive regulation of gene expression,etc.C-type lectin-like receptors,helper T 17(Th17)cell differentiation,tumor necrosis factor(TNF)and interleukin-17(IL-17)signaling pathways were involved.The key targets and the key components of the drug can be successfully docked.Conclusions Kangfu Xiaoyan Suppository exerts its therapeutic effect on chronic prostatitis through multiple components,targets and pathways.
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