Exploration of the mechanism of Morinda officinalis in improving endometrial receptivity based on molecular docking technology
Objective To analyze the mechanism of Morinda officinalis in improving endometrial receptivity by molecular docking technique.Methods A total of 60 Wistar female rats were randomly divided into normal group,model group,clomiphene citrate group and morinda group,with 15 rats in each group.Infertility rat models were constructed in model group,clomiphene citrate group and morinda group.Normal group and model group were given 2 mL normal saline intragastric administration,once a day.Clomiphene citrate group was given 0.83 mg/mL clomiphene citrate solution 2 mL intragastric administration,once a day.Morinda group was given 0.5 g/mL Morinda officinalis solution 2 mL intragastric administration,twice a day.The endometrial thickness,subendometrial blood flow parameters[uterine arterial pulse index(PI),resistance index(RI),systolic/diastolic flow rate ratio(S/D)],and the protein expression levels of endothelial nitric oxide synthase(eNOS)and vascular endothelial growth factor(VEGF)were compared 15 days after administration.Combined with the experimental results,the possible mechanism of action of Morinda officinalis was analyzed by network pharmacology,and the binding sites of active ingredients were predicted by molecular docking.Results Compared with normal group,endometrial thickness,PI,RI,eNOS and VEGF were decreased and S/D was increased in model group(P＜0.05).Compared with model group,endometrial thickness,PI,RI,eNOS and VEGF were increased in clomiphene citrate group,while S/D was decreased in clomiphene citrate group(P＜0.05).Compared with clomiphene citrate group,endometrial thickness,PI,RI,eNOS and VEGF were increased in morinda group,while S/D was decreased in morinda group(P＜0.05).A total of 199 active ingredients of Morinda officinalis were identified by network pharmacology,including 792 endometrial receptivity related targets and 99 intersection targets.Protein-protein interaction(PPI)network analysis of Morinda officinalis mainly acts on ESR1,NCOA1,NCOA2,MAPK14,PGR,AR and other key targets.The results of molecular docking showed that the main active ingredients obtained by screening had strong binding with the target.Conclusions Morinda officinalis may have positive effects on endometrial receptivity,and its mechanism may be related to the regulation of ESR1,NCOA1,NCOA2,MAPK14,PGR and AR signaling pathways.
Molecular docking technologyMorinda officinalisEndometrial receptivityMechanism of action
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NSTL
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