The expression levels of interleukin-35 in M2 subtype macrophages and its effects on the proliferation,invasion and epithelial-mesenchymal transition of prostate cancer cells
Objective To explore the expression levels of interleukin-35(IL-35)in macrophages and its effects and mechanisms on the proliferation,invasion,and epithelial-mesenchymal transition(EMT)of prostate cancer(PCa)cells.Methods The expression levels of macrophage marker CD68,M1 subtype marker tumor necrosis factor-α(TNF-α)and IL-1 β,and M2 subtype marker CD206 and IL-10 in M0,M1 and M2 macrophages after induction and differentiation of human mononuclear/macrophage line THP-1 cells were detected.PCa cell line PC-3 cells were treated with different types of macrophages-sources conditioned medium(CM),and were divided into M0-CM group,M1-CM group,M2-CM group,and M2-CM group(M2-CM+IL-35 NA group)which was pre-interfered with IL-35 neutralizing antibody(IL-35 NA).The proliferation,invasion and EMT of PC-3 cells and the p-JAK2/JAK2 ratio and p-STAT3/STAT3 ratio of JAK/STAT signaling pathway were detected.Results Compared with unstimulated THP-1 cells,CD68 mRNA expression levels were increased in M0,M1 and M2 subtype macrophages(P＜0.01).Compared with M0 subtype macrophages,TNF-α and IL-1 β mRNA were increased in M1 subtype macrophages(P＜0.01),while CD206 and IL-10 mRNA were increased in M2 subtype macrophages(P＜0.01).Compared with M0-CM group,the proliferation,invasion,EMT,intracellular p-JAK2/JAK2 and p-STAT3/STAT3 ratios of PC-3 cells were increased in M2-CM group(P＜0.05).In M2-CM+IL-35 NA group,the proliferation,invasion,EMT,intracellular p-JAK2/JAK2 and p-STAT3/STAT3 ratios of PC-3 cells were significantly decreased(P＜0.05).In M1-CM group,the proliferation,invasion,EMT,intracellular p-JAK2/JAK2 and p-STAT3/STAT3 ratios of PC-3 cells had no significantly difference(P＞0.05).Compared with M2-CM group,the proliferation,invasion,EMT,intracellular p-JAK2/JAK2 and p-STAT3/STAT3 ratios were decreased in M1-CM group and M2-CM+IL-35 NA group(P＜0.05).Conclusions M2 subtype macrophages can promote the proliferation,invasion and EMT of PC-3 cells by secreting IL-35,which may be related to the activation of JAK2/STAT3 signaling pathway by IL-35.
万方数据
维普
