Application of umbilical cord mesenchymal stem cell transplantation in the treatment of uterine incision scar defects after cesarean section in rats
Objective To investigate the application effect of umbilical cord mesenchymal stem cell transplantation in the treatment of uterine incision scar defects after cesarean section in rats.Methods A total of 120 SPF rats with a body weight of 300-350 g were selected.After successful establishing an animal model of uterine diverticulum,rats were divided into A,B,and C groups by random number method,with 40 cases in each group.Group A was given spot injection around the uterine diverticulum and tail vein injection of 0.5 mL normal saline.Group B was given spot injection around the uterine diverticulum and tail vein injection of 0.2 mL umbilical cord mesenchymal stem cell.Group C was given spot injection around the uterine diverticulum and tail vein injection of 0.5 mL umbilical cord mesenchymal stem cell.The continuous treatment lasted for 3 weeks with frequency of once a week.Physiological indexes,serum estradiol(E2),interleukin-6(IL-6)and interleukin-8(IL-8)levels of rats were observed after administration,and the differences of myometrium and endometrium indexes were analyzed at 1 and 4 weeks after treatment.Results Compared with group A,group B and C had increased E2 levels and IL-6 and IL-8 levels decreased,and improvement in group C was more significant than group B(P＜0.05).Groups B and C also showed better improvement in myometrial thickness and actin volume density after treatment,and the effect of group C were more significant(P＜0.05).Moreover,the rate of endometrial defects in groups B and C was lower than in group A,and group C was lower than group B(P＜0.05).Conclusions Umbilical cord mesenchymal stem cell treatment have a positive effect on uterine incision scar defects after cesarean section in rats,which can improve their hormone levels and inflammatory response,and improve the condition of myometrium and endometrium,and high doses(0.5 mL)of umbilical cord mesenchymal stem cells were more effective.
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