Meta-analysis of the relationship between copy number of mitochondrial DNA in embryo culture medium and embryo quality in in vitro fertilization-embryo transfer treatment
Objective To evaluate the correlation between the copy number of mitochondrial DNA(mtDNA)in embryo culture medium and embryo quality in in vitro fertilization-embryo transfer(IVF-ET)treatment through Meta-analysis of Chinese and English literatures systems.Methods The Chinese databases of CNKI,Wanfang,VIP and the English databases of PubMed,Embase and the Cochrane Library were searched for clinical studies on the relationship between the copy number of mtDNA in embryo culture medium and embryo quality in IVF-ET treatment.The search period was from the establishment of the databases to November 2023.The literature quality was evaluated by Cochrane system.The relevant data in the literature were extracted and analyzed by RevMan5.3 software.Results A total of 15 papers(8 Chinese papers and 7 English papers)were included in this study,including 3,542 patients.The results of Meta-analysis showed that the copy number of mtDNA in patients receiving IVF-ET treatment was highly positively correlated with the fertilization rate,high quality embryo rate and embryo score(r=0.726,0.668,0.709;P＜0.000,01),and weakly positively correlated with the cleavage rate(r=0.280,P＜0.030).The results of publication bias analysis showed that the funnel chart distribution of the correlation between the copy number of mtDNA and the fertilization rate,high quality embryo rate,high quality embryo rate and embryo score was basically symmetrical with no publication bias.Conclusions There is a certain correlation between the copy number of mtDNA in embryo culture medium and the embryo quality in IVF-ET treatment.Clinical evaluation of embryo quality can be assisted by detecting the copy number of mtDNA in embryo culture medium,which provides an evaluation basis for clinical process control of IVF-ET treatment.
In vitro fertilization-embryo transferEmbryo culture mediumMitochondrial DNAEmbryo qualityMeta-analysis
万方数据
维普
