Study on the pharmacological mechanism of plantain seed in the treatment of ovarian cancer based on network pharmacology and molecular docking technology
Objective The main active components and potential mechanism of plantain seed(PS)in the treatment of ovarian cancer(OC)were explored by network pharmacology and molecular docking technology.Methods The traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP)and Swiss target prediction database were used to obtain the active ingredients and potential targets of PS.OC-related disease targets were searched with the help of the therapeutic target database(TTD)and gene-disease associations database(DisGeNet).The intersection of drug targets and disease targets was used to draw a Venn diagram.Cytoscape 3.9.1 software was used to construct the"drug-active ingredient-disease-target"network,and the core components were screened out according to their node values.The protein interaction network of the intersecting targets was constructed by using the search tool for the retrieval of interacting genes/proteins(STRING)database,and the core targets were screened out according to the"CytoNCA"plugin of Cytoscape 3.9.1 software.Enrichment analysis of the potential therapeutic targets was conducted with the help of the database for annotation,visualization and integrated discovery(DAVID)database.AutoDock tools software was used to verify the molecular docking between some active ingredients and potential targets.Results MOL000098(quercetin),MOL001663(3-epidulic acid),MOL007819(8-hydroxyluteolin)and other components of PS might act on cancer pathways,chemocarcinogenic-receptor activation pathways,endocrine resistance pathways,cellular senescence pathways,proteoglycan-related cancer pathways,platinum drug resistance pathways and other pathways through key targets TP53,MAPK1,AKT1,ESR1,IL6.The molecular docking results showed that the first two core components and the top five core targets all had good binding ability.Conclusions The treatment of OC with PS may be related to targets like TP53,MAPK1,AKT1,ESR1,and IL6,and pathways like cancer pathways,chemocarnogenic-receptor activation pathways,endocrine resistance pathways,cellular senescence pathways,proteoglycan-related cancer pathways,and platinum drug resistance pathways,among which quercetin may be the main component for it to exert functions.
万方数据
维普
