Degradation Ratio of Chitosan in Rumen and Its Effects on Ruminal Fermentation in Dairy Cows
The present study was conducted to investigate the degradation ratio of chitosan in rumen and its effects on ruminal fermentation in dairy cows. The study included 2 trials. The first experiment was conducted to study the degradability of chitosan in rumen of dairy cows. Three dairy cows, withsimilar age, weight, parity and lactation, were installed permanent rumen fistula to determine the rumen degradability of chitosan at 0, 2, 4, 8, 12, 24, 36 and 72 h by nylon bag technique. The second experiment was conducted to study the effects of different levels of chitosan in substrates (0, 0.02%, 0.1%, 0.5%, 1% and 2% respectively) on in vitro ruminal fermentation. The pH value, concentrations of NH3-N and BCP and TVFA and gas production of in vitro ruminal fluid at 0, 2, 4, 8, 12, 24 h were determined. The results showed that the rumen degradability of chitosan was very low, and it was only about 7.79% at 72 h. The results also indicated that pH value and concentrations of NH3-N and BCP and TVFA and gas production in in vitro ruminal fluid were not affected obviously by chitosan supplementation(P>0.05).