Metabolism of Melamine and Its Effect on Performance of Beef Catle
This trial was conducted to det ermine metabolism of melamine in beef cattle on diets containing melamineand effect on performance of beef cattle.75 Simmental or charolais ×local yellow beef crossbreed weighed about 500 kg were randomly allotted to 5 treatments whichwere fed a diet with 0,2,10,30 and 100 mg/kg melamine,respectively.Melamine concentration of urine were determined on day 7,14,21,28,42 and 56,melamine concentration of plasma were determined on day 14,21,28,42 and 56.Melamine was determined by liquid chromatography with tandem mass spectrometry (LC-MS/MS).The results showed that performance of beef cattle was not affected by supplemental melamine (P>0.05).The residue of melamine in urine,plasmaand dietary melamine concentration was dose independent (P<0.05).Correlation equationbetween dose of melamine and concentration of urine was y=0.0736x+0.1198,R2=0.998.It is concluded that melamine excreted mainly by urine,intake of melamine can be estimated by concentration of melamine in urine.