Cloning,Expression and Bioinformatics Analysis of the CDS Region of Chicken SLMO2 Gene
This study is to investigate the amino acid sequence characteristics of drosophila-like slow-moving gene and its mRNA expression in different tissues of chicken with different skin colors.The CDS sequence of SLMO2 with a total length of 560 bp was cloned by PCR,and it encoded 174 amino acids.The bioinformatics analyses showed that there were no transmembrane structures and signal peptides in the chicken SLMO2 protein,and the protein structure was mainly composed of irregular curly and α-spiral structures,and it was distributed in the mitochondrial inner membrane space.Amino acid sequence comparison showed that the genetic relationship between chicken and Xenopus was close.qRT-PCR assay showed that the expression level of SLMO2 in the back skin tissue of Silkie was significantly higher than that of other tissues(P<0.01),and the expression level of SLMO2 in the back skin tissue of yellow-feather chicken was significantly higher than that in other tissues(P<0.01).The above results will provide a theoretical basis for further study on the regulatory mechanism of chicken SLMO2 gene in melanin deposition.
ChickenSLMO2 geneCDS region cloningExpression analysisBioinformatics analysis
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