Research Progress on Donkey Semen Cryopreservation
Semen freezing technology is of great significance in the long-term preservation and introduction of donkey germplasm resources,as well as the application of artificial assisted reproduction technology.Semen freezing storage is conducive to fully exerting the reproductive power of excellent male donkeys and reducing the production cost of donkey breeding industry.In recent years,donkey semen cryoprotectants and freezing methods have been optimized continuously,and great progress has been made in donkey semen cryopreservation technology.However,due to the low sperm viability and conception rate of donkey cryoresuscitation,the promotion and application of donkey frozen semen are severely limited.This article reviews the development of donkey semen cryopreservation technology,the mechanism of sperm cryopreservation damage,the factors affecting donkey semen cryopreservation,and the application of donkey frozen semen,providing reference for future research on donkey semen cryopreservation technology.