Research Progress on Sexed Sperm Sorting and the Differential Proteins in Mammals
Sex sorting of mammalian spermatozoa is crucial for pre-fertilization sex control.After artificial insemination,the sexed sperm will be combined with oocyte for production of the progeny with predicted sex.In recent years,approaches of sperm sex-sorting have been discussed and optimized,and multiple methods have been obtained,including flow cytometry,Percoll® gradient centrifugation,H-Y antigen,Sephadex gel filtration,etc..However,none of them could achieve 100%accurate sperm sex-sorting.In this paper,we reviewed the research progress on the biophysical characteristics of sexed sperm,the current status of commercial sperm sex-sorting technologies,and the different proteins of sexed sperm,which will provide theoretical reference for further research and optimization of mammalian sperm sex-sorting technology and its popularization and application.
X spermY spermFlow cytometry sortingKits sortingDifferential proteins
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