Effect of Jian-Pi-Zhi-Dong decoction on autonomous activity and dopamine synaptic vesicle protein expression in the striatum of Tourette syndrome model rats
Objective To explore the effect of Jian-Pi-Zhi-Dong decoction on autonomous activity and dopamine(dopamine,DA)synaptic vesicle protein expression in the striatum of Tourette syndrome(TS)model rats.Methods The 4-week-old male SD rats were used to establish the TS model by intraper-itoneal injection of N-aminodipropionate.Thirty successfully modeled rats were randomly divided into model group,Chinese medicine group,and tiberide group according to random number table method,with 10 rats in each group.And another 10 rats with matched body mass were selected as the control group.Rats in Chinese medicine group were given Jian-Pi-Zhi-Dong decoction solution(1.6 g/100 g)and the rats in tiapride group were given sulfate tiapride suspension(2.1 mg/100 g),while rats in control group and model group were given an equal volume of 0.9％sodium chloride solution,once a day for 4 weeks.The number of autonomous activities in rats was determined by autonomous activity programmer.ELISA was used to detect the level of DA in the striatum of rats and the expression of dopamine transporter(DAT).Vesicular monoamine trans-porter-2(VMAT2)and α-synuclein(α-syn)were measured by Western blot.SPSS 25.0 software was used for data analysis.Multiple group comparisons were performed using one-way ANOVA,non-parametric test and repeated measures ANOVA.Results Comparing the number of autonomous activities among the 4 groups,the interaction effect between time and group was significant(F=184.354,P＜0.001).At the 1-4 weeks of gavaging,the numbers of autonomic activities in the model group were more than those in the control group(all P＜0.05).While the numbers of autonomic activities in Chinese medicine group and tiapride group were less than those in the model group(all P＜0.05).Moreover,the numbers of autonomic activities in Chinese medicine group and tiapride group from 1 to 4 weeks were less than those after model making(all P＜0.05).The Western blot results showed significant differences in the relative expression of α-syn(H=29.098),DAT(F=54.632)and VMAT2(H=18.982)among the 4 groups(all P＜0.001).The expres-sion levels of α-syn protein in Chinese medicine group and tiapride group were both lower than that in the model group(0.39(0.36,0.51),0.39(0.36,0.50),0.62(0.50,0.70))(both P＜0.05).The expression level of DAT protein in Chinese medicine group was higher than that in the model group and lower than that in tiapride group((0.37±0.06),(0.26±0.07),(0.49±0.09))(both P＜0.05).And the expression lev-el of VMAT2 protein in Chinese medicine group had no significant difference compared with that in the model group(P＞0.05).The ELISA results showed significant differences in DA content of striatum among the 4 groups(F=75.370,P＜0.001).The level of DA in the model group was higher than that in the control group((7.65±0.72)ng/L,(3.71±0.59)ng/L,P＜0.05).The levels of DA in Chinese medicine group((3.92±0.81)ng/L)and tiapride group((4.40±0.53)ng/L)were lower than that in the model group(both P＜0.05),and the difference between Chinese medicine group and tiapride group was not significant(P＞0.05).Conclusion Jian-Pi-Zhi-Dong decoction can relieve the tic symptoms of the model rats with TS,and its mechanism may be related to inhibiting the excessive release of α-syn,improving the expression of DAT and VMAT2,improving the DA synaptic vesicle circulation,and reducing the DA content in the synaptic space of the brain.
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