Objective To study the role and relationship between conventional cytogenetic(CC)and multiplex fluorescence in situ hybridization(FISH)in the diagnosis of lymphoma.Methods 30 patients with lymphoma in First Affiliated Hospital of Soochow University were examined by CC and multiplex FISH simultaneously.The chromosomal abnormalities detected by the two methods were analyzed to clarify the relationship between the two methods in the diagnosis of lymphoma.Results A considerable portion of the chromosomal abnormalities identified by CC were complex types,with an abnormality detection effect of 53.6%.The abnormality detection effect obtained by the multiplex FISH was 43.3%.The P value of paired chi-square test was 0.58＞0.05,indicating that there was no significant difference in the detection rate of chromosomal abnormalities between the two methods.Same chromosomal abnormalities of some samples were detected by CC and multiplex FISH simultaneous.CC could detect chromosomal abnormalities outside the scope of the probe of multiplex FISH,and multiplex FISH could detect chromosomal abnormalities in the samples where CC failed or did not detect.Conclusion CC and multiplex FISH complement each other,and multiplex FISH can expand the information of FISH in one detection.Both of them provide important genetic data for the diagnosis and treatment of lymphoma.
关键词
荧光原位杂交/常规染色体检测/淋巴瘤/细胞遗传学异常
Key words
fluorescence in situ hybridization/conventional cytogenetic/lymphoma/cytogenetic abnormalities
维普
万方数据