Effects of Platycodon grandiflorum extract on proliferation and metastasis of rheumatoid arthritis fibroblast-like synovial cells based on RhoA/ROCK pathway
AIM To investigate the effects of Platycodon grandiflorum extract(PGE)on the proliferation and metastasis of fibroblast-like synovial cells in rheumatoid arthritis(RA)and its mechanism of action.METHODS Fibroblast-like synovial cells MH7A were divided into control group,PGE-L group(25 mg·L-1 PGE),PGE-M group(50 mg·L-1 PGE),PGE-H group(100 mg·L-1 PGE),PGE+angiotensin(Ang)Ⅱ group(100 mg·L-1 PGE+100 nmol·L-1 Ras homologous gene family member A(RhoA)/Rho kinase(ROCK)pathway activator Ang Ⅱ),and treated accordingly.Cell proliferation,apoptosis,migration and invasion were detected by CCK-8 method,flow cytometry and Transwell,respectively.The expression levels of related proteins were detected by Western blot,and the RhoA activity in cells was detected by GST-pull down method.RESULTS Compared with the control group,absorbance value(24,48 and 72 h after PGE treatment),the number of migrating and invading cells,the expression of cyclin D1,matrix metalloproteinase(MMP)-2,MMP-9,ROCK1 and ROCK2 proteins,and the activity of RhoA were significantly reduced,and p21 protein expression and the apoptosis rate were increased in PGE-L group,PGE-M group,and PGE-H group,and the higher the concentration of PGE,the more obvious the corresponding trend was(P＜0.05).Compared with the PGE-H group,the absorbance value(24,48,72 h after PGE treatment),the number of migratory and invasive cells,the expression of cyclin Dl,MMP-2,MMP-9,ROCK1,ROCK2 protein and RhoA activity were increased,amd p21 protein expression and apoptosis rate decreased in PGE+Ang Ⅱgroup(P＜0.05).CONCLUSION PGE can inhibit the proliferation and metastasis of MH7A cells,which may be related to the inhibition of RhoA/ROCK pathway.
Platycodon is radixarthritis,rheumatoidsynoviocytesneoplasm metastasisproliferationPlatycodon grandiflorum extract
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