AIM To investigate the protective mechanism of berberine against Polygonum multiflorum thunb.induced liver injury.METHODS SD rats were randomly divided into sham group,PM(Polygonum multiflorum thunb.40 g·kg-1)group,PM+NS(saline 1 mL)group,PM+BBR(berberine 50 mg·kg-1)group(n=12 in each group).Another 24 rats were divided into PM+BBR+sh-PPAR-γ group and PM+BBR+sh-NC group,which were injected with 100 μL of shRNA-PPAR-γ and 100 μL of shRNA-NC into the tail vein.The administration of each group lasted for 8 weeks.HE staining was used to observe the pathological changes in liver tissue,and serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP)levels and the levels of IL-1β,IL-6 and IL-10 in liver tissues were detected by ELISA.PPAR-γ expression was knockdown by transfecting shRNA-PPARγ into rats.The expression levels of PPAR-γ in rat liver tissues was detected by RT-qPCR and Western blot.RESULTS Compared with the sham group,the levels of serum ALT,AST,ALP were significantly increased,the levels of IL-1β,IL-6 in liver tissue were increased and IL-10 level was decreased,and PPAR-γ expression was significantly downregulated in the PM group and PM+NS group(all P＜0.05).Compared with the PM+NS group,serum ALT,AST,ALP levels were significantly decreased,IL-1β and IL-6 levels were significantly decreased,IL-10 level was significantly increased,and PPAR-γ expression was significantly increased in the PM+BBR group(all P＜0.05).Compared with the PM+BBR+sh-NC group,PPAR-γ expression was significantly decreased,serum ALT,AST,ALP levels were significantly increased,IL-1β and IL-6 levels were significantly increased,IL-10 level was significantly decreased in the PM+BBR+sh-PPAR-γ group(all P＜0.05).CONCLUSION Berberine may alleviate hepatic injury induced by Polygonum multiflorum thunb.by up-regulating PPAR-γ expression.
chemical and drug induced liver injuryberberinePPAR gammainflammation
NETL
NSTL
万方数据
