Salidroside regulates cell proliferation,migration and collagen synthesis of human keloid fibroblasts through Notch1/Hes1 pathway
AIM To investigate the effects of salidroside(Sal)on the proliferation,migration and collagen synthesis of human keloid fibroblasts(HKF)by regulating Notch 1/Hes1 pathway.METHODS HKF in logarithmic growth period was divided into control group(NC group),Sal-L,Sal-M,Sal-H(10,20,40 μmol·L-1)group,Jagged1 group(Notch activator,5 mg·L-1),Sal(40 μmol·L-1)+Jagged1(5 mg·L-1)group.MTT assay was used to detect cell proliferation.Cell cycle and apoptosis were detected by flow cytometry.Cell migration was detected by scratch test,and Western blot was used to detect the expression of collagen-Ⅰ,collagen-Ⅲ,proliferating cell nuclear antigen(PCNA),matrix metalloproteinase-2(MMP-2),Notch1 and Hes1 proteins.RESULTS Compared with the NC group,the A value,the proportion of cells in S phase and G2/M phase decreased,scratch healing rate decreased,the protein expression of collagen-Ⅰ,collagen-Ⅲ,PCNA,MMP-2,Notch1 and Hes1 decreased in the Sal-L,Sal-M and Sal-H group,the proportion of cells in G0/G1 phase and the rate of apoptosis increased,in a dose-dependent manner(P＜0.05),while the Jagged1 group showed the opposite change of the above indexes.Compared with the Sal-H group,the A value,the proportion of cells in S phase and G2/M phase,scratch healing rate,the protein expression of collagen-Ⅰ,collagen-Ⅲ,PCNA,MMP-2,Notch1 and Hes1 increased,the proportion of cells in G0/G1 phase and the rate of apoptosis decreased in the Sal group(P＜0.05).CONCLUSION Sal may inhibit the proliferation,migration and collagen synthesis of HKF by inhibiting Notch1/Hes1 pathway.
万方数据
维普
