Chemical constituentss, odor types and general smoking quality of 112 samples from 2007 to 2009 seasons were analysed to study the effect of main chemical constituents on smoking quality of flue-cured tobacco. Results showed that (1) the fresher the scent of leaf, the better the smoking quality. (2) for leaf with fresher scent, content of nicotine and nitrogen were relatively low, while the ratio of K/Cl was high. (3) content of nicotine, nitrogen, reducing sugar, and total sugar were main factors affecting smoking quality. (4) the optimum content of nicotine and total nitrogen were 1.4%-2.0% and 1.4%-1.8%, respectively. Based on the research, moderate control of nicotine and total nitrogen level was proposed to improve the fresh flavor.