Effects of water stress on ammonium assimilation and biomass of flue-cured tobacco
Difference of ammonium assimilation and nitrogen metabolism under different water conditions and hence effects of drought and water logging on key enzyme activities and plant growth were studied with pot experiment using cultivar Zhongyanl00. Results showed that GS and GOGAT activities in leaves decreased and the GS/GOGAT cycle was inhibited under drought at vigorous growing period, while NADH-GDH activities increased indicating it played a more important role in ammonium assimilation. Tobacco plant growth was restrained and plant biomass decreased under drought, which was the reason for nitrogen content increase. GS activities rose rapidly and proline content recovered after re-watering, which was a compensation effect to some extent. Water logging caused leaf metabolic disturbance and hence key enzyme activities, nitrogen content and plant biomass all significantly decreased, an adverse condition that cannot be ignored.
flue-cured tobacco (Nicotiana tabacum L. )waterammonium assimilationenzyme activity
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