Effect of composition diff erences of alkaloid in burley tobacco leaves on TSNAs formation before and after high temperature storage
Field experiment was set up to select tobacco samples with different alkaloid composition by choosing different nicotine conversion strains of TN86 under the same fi eld management and air-curing conditions. The selected samples were stored in temperature/humidity-controlled chamber at 45℃ for 12 d to explore the source of TSNAs accumulation during high temperature storage and its relationship with alkaloid content. Results showed that signifi cant variation of individual alkaloids existed among 90 tobacco plants with greatest diff erences being observed for nicotine and nornicotine contents. With approximately same levels of total alkaloids, NO3-N and NO2-N contents, TSNAs contents at 45℃ for 12 d were signifi cantly higher than those before storage. The increase of NNN and NNK contents during storage was significantly positively correlated with their precursors, nicotine and nornicotine, respectively. With the increase of nornicotine content, the net increase of NNN from nornicotine nitrosation during storage was greater than the net decrease of NNK from nicotine nitrosation, leading to the net increase of total TSNAs content. Results of correlation and stepwise regression analysis showed that the increased percentage of nornicotine caused by nicotine conversion, was the main factor infl uencing TSNAs formation during high temperature storage. It could be concluded that at same levels of total alkaloid, NO3-N and NO2-N content of tobacco leaves, the diff erence in alkaloid composition has signifi cant impact on TSNAs formation and accumulation during leaf storage.
国家科技期刊平台
NSTL
万方数据
维普
